Moreover, another likely reason behind this apparent difference in the sero-prevalence of ovine toxoplasmosis detected by LAT (44.5%) and optimized ELISA kit (35.5%) might be the fact that both types of antibodies i.e. IgM and IgG can interact with Toxoplasma total antigenic extract used in the LAT as against the sole detection of IgG in the newly optimized ELISA kit using a single recombinant antigen. Here, LAT is linked to toxoplasmosis.