Out of 400 sheep samples, 168 (42%) samples were found as seropositive for toxoplasmosis by LAT (Pearson Chi-square (χ2) value 275.156; p < 0.0001) and 133 (33.25%) as seropositive through optimized ELISA (Pearson Chi-square (χ2) value 86.049; p < 0.0001) (Table 4). This evidence concerns the gene LAT and toxoplasmosis.