In addition, this study also compares the efficacy of probiotic and ACT in mitigating the severity of comorbid depression and anxiety symptoms; decreasing serum level of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α; changing the event-related potential in EEG and restoring microbiota in the gut of AUD patients. Here, IL1B is linked to depressive disorder.