However, we also observed an increase over time in the N/C ratio of GFP-TFEB in macrophages that engulfed stationary-phase Salmonella; at 4 and 6 h post-engulfment, the N/C ratio of GFP-TFEB in these cells was higher than resting cells or those at 1 h post-infection, and comparable to macrophages infected with E. coli (Fig. 1C and D). The gene discussed is TFEB; the disease is infection.