On the other hand, cumulative evidence supports the use of L1CAM as a target for the isolation of blood-borne nEVs, including the enrichment of EV and neuronal markers in L1CAM IP eluates from human blood as well as the correlation of EV cargo with brain disease states and therapeutic interventions [54, 76–84], the recovery of GFP-positive EVs from the plasma of Nestin-GFP mice selectively expressing GFP in neurons [79], and the positive correlations between brain and nEV levels of pathological proteins in multiple AD mouse models [43]. This evidence concerns the gene L1CAM and Alzheimer disease.