To investigate the degree to which FL to DHL transformation alters ZCCHC7 expression, we performed immunohistochemistry for ZCCHC7 using tissue microarrays (TMAs) containing 33 DLBCLs and 9 DHL samples and using pairs of lymphomas representing FL transformation to DLBCL from nine patients (these stained pairs were distinct from our sequenced DHL cohort; Extended Data Fig. 8a,b). This evidence concerns the gene ZCCHC7 and diffuse large B-cell lymphoma.