In this study, analysis of the Creatinine, protein carbonyl, 8-OHdG, VEGF, S.O.D., G.S.H., C.A.T., NO, MMP-9 Inhibitor, and IL-10 was carried out to investigate the nickel-induced biochemical changes in colon cancer cells. This evidence concerns the gene MMP9 and malignant colon neoplasm.