To investigate the roles of the PI3K/AKT/mTOR pathway in cancer cells when exposed to a high-POSTN environment, we cultured HEYA8 cells in 3D with either CMPOSTNhigh or CMCTL medium, CMCTL + MK2206 (AKT inhibitor), or CMPOSTNhigh + MK2206 for 72 h. This evidence concerns the gene MTOR and cancer.