Western blot analysis of HCC tissue from patients found that in patients 1, 2, 3, 4, and 6, the expression of CPA4 protein was positively correlated with p-AKT, while it was negatively correlated with PP2A expression and GSDME cleavage, which is consistent with the cellular research in this study (Fig. S6d). Here, GSDME is linked to hepatocellular carcinoma.