In our research, EZH2 and RUNX3 expressions in colon carcinoma cell strains were examined at the cytological level, verified the effects of EZH2 upon their migration, proliferation, apoptosis, and invasion were verified, and the molecular mechanism of EZH2 action was further explored so as to provide new targets for targeted therapy of colon cancer. Here, RUNX3 is linked to malignant colon neoplasm.