Next, to examine the cooperation of p53 alterations and diet to drive liver cancer formation, we generated three cohorts: one cohort deletes one allele of Trp53 specifically in hepatocytes (p53fl/+, Alb-CreTg; labeled LPfl/+ for liver specific Trp53 deletion), one has a somatic p53 missense mutation R245W specifically in hepatocytes (p53wm245/+, Alb-CreTg; LP245/+), and the third has no alterations (p53+/+, Alb-CreTg; LP+/+). Here, TP53 is linked to liver cancer.