Induction of Clostridium ramosum-specific IgG and IgA. Lower C. ramosum levels in the colonic mucosa of vaccinated mice compared to those of naïve mice and mice immunized without antigen Mice vaccinated with antigen showed significantly lower weight gain, lower epididymal and mesenteric white adipose tissue, decreased Slc2a2 levels in the ileal epithelium, and blood glucose levels than control animals, despite similar food intake, suggesting a preventive effect of vaccination on C. ramnosum-mediated obesity. Here, SLC2A2 is linked to obesity due to melanocortin 4 receptor deficiency.