Among the upregulated cytokines, the levels of CCL9, CXCL16, TIMP-1, IGF-BP-3, CXCL2, and sTNFRII were elevated both in spleen and tumor tissue (Fig. 4A) (the spleen chip was previously published).20GEPIA database showed that CCL15 (human ortholog of mouse CCL9), CXCL16, and TIMP-1 were increased in HCC patients, whereas IGF-BP-3, CXCL2, and sTNFRII were decreased in HCC patients (Fig. 4B). This evidence concerns the gene TIMP1 and hepatocellular carcinoma.