All the cellular changes that we detected after 24 and 48 h of embedding into the hydrogel at higher stiffness, such as morphology, expression of the cyclin D1, histone H3 acetylation as well as the different sensitivity to H2S, which was reported to increase cell viability of the normal or mesenchymal stem cells [20,27,35,65,66], are in agreement with a physically induced trans-differentiation (PiT) of the cancer cells. This evidence concerns the gene CCND1 and cancer.