Predicted binding affinities of peptide epitopes from these genes to the relevant H2Kb and H2Db MHC Class I molecules of C57Bl/6 mice identified several strong binding epitopes from the Lcn2, Lect2, Smagp, Nsdh1 and Plrg1 genes (Fig. 5B–C), suggesting that over-expression of these genes in Sleeping Beauty HCC may expose potential neo-antigens for endogenous CD8+ T cell priming/recognition. This evidence concerns the gene PLRG1 and hepatocellular carcinoma.