We further validated antigen-specificity in the YT-rCTL/K562-FRET system using a melanoma-reactive TCR, clone 103 from patient EB81, here shortened to EB81.103 (28), responsive to a previously characterized peptide epitope (EVDPIGHLY) derived from the human MAGEA3 protein presented in the context of the HLA-A*01:01 allele. Here, MAGEA3 is linked to melanoma.