On the other hand, we focused on six genes (COL4A5, GLIS3, NPR1, OSR2, PLAGL1 and RSPO3) that were significantly downregulated in human prostate tumors as well as upregulated in DU145-R427 cells, suggesting that these genes might negatively interfere with prostate tumorigenesis. The gene discussed is RSPO3; the disease is prostate neoplasm.