Bioinformatic analyses (Supplementary Figure 4A) indicated that genes upregulated in R427 cells (COL4A5, GLIS3, NPR1, OSR2, PLAGL1, RSPO3) were all significantly downregulated in human prostate adenocarcinomas, while genes downregulated in R427 cells were either significantly higher expressed (CBLC) or trending towards enhanced expression (METTL27) in prostate tumors. The gene discussed is RSPO3; the disease is prostate neoplasm.