Furthermore, RT-qPCR (Fig. 1E) and western blot (Fig. 1F, G) analyses of SIRT1 expression in HCC cell lines (Huh7, HepG2, SNU-423, SNU-398, and HCCLM3) showed that SIRT1 expression was significantly elevated in HCC cell lines (p < 0.001). This evidence concerns the gene SIRT1 and hepatocellular carcinoma.