This study aimed to investigate the role of SIRT1 and GSDME-related pyroptosis in HCC using various techniques, including immunohistochemistry, western blotting, and quantitative reverse transcription polymerase chain reaction, as well as cell counting kit-8, wound healing, transwell invasion, flow cytometry, and lactate dehydrogenase release assays. Here, GSDME is linked to hepatocellular carcinoma.