On the one hand, low-frequency variants such as I﻿DH1 (Arg13﻿﻿2G﻿ly﻿), TP53 (c﻿.﻿﻿919 + 1G > ﻿A﻿), GNAS (Ar﻿g201Cys) and MSH (Try1066Cys), as well as high-frequency variants  PTCH1(c.1347 + 6G > A) and ALK (Ser691Ser) detected in the plasma of patients were not found in the matched tumor. Here, PTCH1 is linked to neoplasm.