EGF and neoplasm: Briefly, the authors utilized WNT3A, EGF, NOGGIN, and RSPO1 (WENR) supplemented medium and mechanically processed tumor fragment growing in air–liquid interface to establish PDOs from 100 individual patients with different subtype and localization of cancers, including 20 colorectal cancers, 11 pancreas, 20 kidney, 20 lung and 29 other tumor types.