Herein, we aims to analyze the changes in bone microarchitecture and mineralization over time by microcomputed tomography (micro-CT) in a mouse model of NAFLD induced by a high-fat diet (HFD) and hereby address the following aims: (1) investigate the changes of bone microstructure and pathogenesis in the progression of NAFLD; (2) investigate the role of TNF-α and IGF-1 during osteoporosis in NAFLD mice. The gene discussed is IGF1; the disease is osteoporosis.