SP7 and metabolic dysfunction-associated steatotic liver disease: From 24 to 32 weeks, the mRNA levels of OPG, OSX and Runx2 in tibiae of HFD group were decreased in comparison to the WT group, while the RANKL mRNA level showed opposite results (P < 0.01, Fig. 6E–H), indicating that osteoblast function was gradually weakened with the aggravation of NAFLD.