Herein, we aims to analyze the changes in bone microarchitecture and mineralization over time by microcomputed tomography (micro-CT) in a mouse model of NAFLD induced by a high-fat diet (HFD) and hereby address the following aims: (1) investigate the changes of bone microstructure and pathogenesis in the progression of NAFLD; (2) investigate the role of TNF-α and IGF-1 during osteoporosis in NAFLD mice. Here, IGF1 is linked to metabolic dysfunction-associated steatotic liver disease.