Furthermore, we also analyzed the expression intensity of MSI2, HSPB1, p-HSPB1(Ser78) and ACSL4 in our clinical CRC samples by IHC, and found that MSI2 expression was positively correlated with HSPB1 and p-HSPB1(Ser78) expression, but negatively correlated with ACSL4 expression (Fig. 8D), which was confirmed by the positive association between MSI2 and HSPB1 gene effect scores in CRC cell lines from DEMETER2 (R = 0.26) (Fig. 8E). This evidence concerns the gene ACSL4 and colorectal carcinoma.