To determine whether MSI2 affects CRC cell proliferation, we first performed CCK-8 assays to assess the viability of stable cells after MSI2 knockdown in SW620 and LOVO cells or overexpression in HT29 cells and found that MSI2 increased the viability of stable cells in vitro (Fig. 2C). This evidence concerns the gene MSI2 and colorectal carcinoma.