MSI2 and colorectal carcinoma: Consistent with this finding, an immunofluorescence assay with JC-1 staining of stable SW620 and LOVO cells confirmed that the JC-1 aggregate (PE+) intensity was decreased after MSI2 knockdown; the shift from aggregates to monomers indicates the loss of MMP in MSI2-deficient CRC cells (Fig. 2J and K).