Our analysis of COAD tissues and normal tissues in the ENCORI database showed that NFKBIA (IκBa) was lowly expressed in colon cancers, and IL6 and IL8 were highly expressed in colon cancers (Fig. 7C), which suggests that the downstream regulatory molecules of UBE2D3 play an important role in the development of tumorigenesis. This evidence concerns the gene IL6 and malignant colon neoplasm.