However, due to the impact of funds, sample storage and logistics during the COVID-19 pandemic, this study could not further determine the protein expression levels of AKT1 and MAP2K1 genes in blood, nor could it conduct a complete detection of chemokines in the chemokine signaling pathway in blood, so it could not fully explore their role and mechanism in the pathogenesis of AD or MCI, which were limitations of this study. Here, MAP2K1 is linked to Alzheimer disease.