At 1.5 h (1–2 h), radioligands[64Cu]Cu-1 and [64Cu]Cu-2 showed a low uptake(SUVmax ∼ 1–1.5) in the PC3 PD-L1-overexpressingtumor, with negligible to little contrast between target-overexpressingand mock tumor. Here, CD274 is linked to neoplasm.