Data obtained were in line with our findings in breast tumor tissue, i.e., CSC (ALDH1A1) and Tregs (FOXP3) markers possessed a positive correlation in glioma (dataset: Tumor Brain Lower Grade Glioma (2022-v2) - tcga − 532 - tpm - gencode36) (r = 0.1515, p < 0.001, n = 532) and prostate adenocarcinoma (dataset: Tumor Prostate Adenocarcinoma-TCGA-497- rsem–tcgars) (r = 0.1529, p < 0.001, n = 497) (Fig. 1A right panel). This evidence concerns the gene FOXP3 and prostate adenocarcinoma.