NANOG and breast neoplasm: Third, to broaden the scope of our data, we performed immunohistochemistry (IHC) of breast tumor patient-derived tissues, which revealed an augmented incidence of stemness factors (OCT4, SOX2, NANOG) along with Treg signature gene FOXP3 (Fig. 1C) in high-grade (n = 4) than low-grade (n = 4) breast tumor tissues.