To that end, in our third approach, in-silico Kaplan-Meier data of NACT-treated breast cancer patients showed positive correlation between higher FOXP3 expression (p < 0.05) (Online Resource 4, Supplementary Fig. 2C) with lower RFS, as compared to NACT patients expressing lower FOXP3, reflecting the correlation pattern we found with higher stemness (OCT4, NANOG) and lower RFS (Online Resource 4, Supplementary Fig. 2A and B) under similar conditions. This evidence concerns the gene NANOG and breast carcinoma.