To that end, supporting our hypothesis, in-silico RNA profiling database from ‘R2: Genomics Analysis and Visualization Platform’ showed a positive correlation between CSC marker ALDH1A1 and TGFβ1 (GSE 69,031) (r = 0.1863; p < 0.05) (Fig. 4A left panel) as well as Treg signature FOXP3 and TGFβ1 (GSE5460) (r = 0.2955; p < 0.001) (Fig. 4A right panel) in breast cancer patients. This evidence concerns the gene TGFB1 and breast cancer.