As a step to generalize our findings and considering the clinical relevance of DAXX and ATRX defects in human pancreatic neuroendocrine tumors (PanNETs) (67,68), we next examined centromeric DSB accumulation in the panNET cell line BON-1, as well as in ATRX-KO and DAXX-KO derivatives thereof, which we generated by CRISPR, and in DAXX-KO BON-1 cells expressing WT DAXX, DAXX (Y124A) or DAXX (Y124A, R371W) (Figure 6C). This evidence concerns the gene DAXX and pancreatic neuroendocrine tumor.