Since mutations affecting the H3.3/ATRX/DAXX axis are frequently observed in pediatric high grade glioma (pHGG) (52), we chose the pHGG-derived adherent cell line SF188 (H3.3/ATRX/DAXX WT, p53 mutant) to generate an experimental system for the identification of novel ATRX-independent functions for DAXX. The gene discussed is ATRX; the disease is central nervous system cancer.