They demonstrated a change in gut microbiome independent of obesity by knocking out resistin-like molecule beta (RELMβ) in mice, a colonic goblet cell-specific gene whose expression is heavily dependent on the gut microbiome, and subjugated the mice to a high-fat diet (HFD), which normally induces RELMβ expression [36]. This evidence concerns the gene RETNLB and obesity due to melanocortin 4 receptor deficiency.