In PDO15 datasets, the clusters representing Ki67+ proliferative, CD34+ endothelial, and IBA1+/CD3+ immune cells have been observed as impaired in PDO samples in comparison with the patient tumor (Fig EV5E, Table 6), confirming the loss of these cell types observed in PDOs of tumor #2 by immunofluorescence (Figs 3D′′ and EV3A′). The gene discussed is AIF1; the disease is neoplasm.