As shown in Fig. 4D–G, compared to WT mice, Lrg1−/− mice exhibited increased protein levels of cell adhesion-related molecules, including Cldn11 (Fig. 4D), Anxa2 (Fig. 4E), Pcdh9 (Fig. 4F), and Itgb5 (Fig. 4G), in endothelial cells after cerebral ischemia‒reperfusion injury. This evidence concerns the gene ANXA2 and brain ischemia.