As shown in Fig. 4D–G, compared to WT mice, Lrg1−/− mice exhibited increased protein levels of cell adhesion-related molecules, including Cldn11 (Fig. 4D), Anxa2 (Fig. 4E), Pcdh9 (Fig. 4F), and Itgb5 (Fig. 4G), in endothelial cells after cerebral ischemia‒reperfusion injury. The gene discussed is ITGB5; the disease is brain ischemia.