LRG1 and Cerebral ischemia: To investigate the impact of Lrg1 knockout on various cells following cerebral ischemia‒reperfusion injury, we conducted single-cell RNA sequencing (scRNA-seq) on cells obtained from the brains of WT sham-operated mice (sham + WT group), Lrg1−/− sham-operated mice (sham + Lrg1−/− group), WT mice after MCAO/R (MCAO/R + WT group), and Lrg1−/− mice after MCAO/R (MCAO/R + Lrg1−/− group) (Fig. 3A).