Through comparison of subcluster fractions in two AD models to age-matched WT controls (3 months old), we detected 16 and 14 significantly changed subclusters (FDR of 5%, at least twofold change) in the EOAD (5xFAD) model and LOAD (APOE*4/Trem2*R47H) model, respectively (Fig. 4a and Supplementary Tables 10 and, 11). This evidence concerns the gene APOE and Alzheimer disease.