Here, using tumoroids and normal adjacent organoids from patients 8 and 24, we observe that CAFs have limited ability to induce SPP1+ macrophage polarization relative to carcinoma cells, and that the presence of both carcinoma cells and CAFs elicits the strongest SPP1+ macrophage polarization (Supplementary Fig. 10C–E). The gene discussed is SPP1; the disease is carcinoma.