To this end, the primary myoblasts isolated from the Myodf/f mice were induced to differentiation for 48 h and then infected with adenovirus expressing Cre (Ad-Cre) to achieve deletion of Myod (cKO), infection with the adenovirus expressing EGFP (Ad-EGFP) as controls (cWT) (Supplementary Fig. 3c). This evidence concerns the gene MYOD1 and infection.