TPM1, a circNEB‐peptide interaction protein, highly expressed in muscle tissue, plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction.[20] Mutations in the TPM1 gene cause hypertrophic cardiomyopathy.[21] In this study, co‐IP assay detected that circNEB‐peptide combined with TPM1 protein, which promoted the differentiation of muscle fibers and the expression of MyoD, MyoG, and Myhc muscle differentiation genes. This evidence concerns the gene MYH6 and hypertrophic cardiomyopathy.