Another study on AOM/DSS CRC mice discovered that treatment with anti-CTLA-4 and lysates of Lactobacillus acidophilus reduced the amount of tumors and Tregs and M2 macrophages in mesenteric lymph nodes and increased the amount of IL-2 and IFN-γ in the serum, and CD8+ T-cell infiltration in the tumor (Zhuo et al., 2019). This evidence concerns the gene CTLA4 and colorectal carcinoma.