To investigate the role of myeloid PFKFB3 in renal fibrosis, we generated myeloid-specific Pfkfb3-deficient mice (Pfkfb3ΔMφ) and their controls (Pfkfb3WT) by breeding floxed Pfkfb3 mice with Lysm-Cre mice (Supplementary Figures 3A, B). The gene discussed is PFKFB3; the disease is renal fibrosis.