We performed quantitative polymerase chain reaction experiments in 14 IPF specimens and 10 normal specimens to verify the bioinformatic results, which showed that genes such as C6orf105, ANK3, MBNL1, LYRM7, and AKAP9 were significantly higher in normal samples than in IPF samples, with the most significant differences in the expression being of ANK3 and LYRM7 (Figure 12A). The gene discussed is AKAP9; the disease is idiopathic pulmonary fibrosis.