To test this hypothesis, we treated Hdac3-LKO mice with ferrostatin-1 (Fer-1), which selectively blocks ferroptosis by inhibiting lipid peroxidation and preventing damage to lipid membranes [36,42] .We found that compared to vehicle-treated Hdac3-LKO mice, Fer-1–treated Hdac3-LKO mice had significantly lower levels of serum ALT (Fig. 4H) and AST (Fig. 4I) as well as reduced Sirius red (Fig. 4J) and Masson’s trichrome (Fig. 4K) staining of liver sections, indicating that Fer-1 treatment reduces liver fibrosis in Hdac3-LKO mice. This evidence concerns the gene HDAC3 and Hepatic fibrosis.