The five most prominent differentially expressed prognostic T2DM-PRGs targeted by miRNAs were BRD4, HSP90AB1, PTEN, PKN2, and VIM. The top five differentially expressed T2DM-PRGs regulated by TFs were HSP90AB1, VIM, PLCG1, SCAF11, and PTEN. Phosphatase and tensin homolog (PTEN) activates the protein kinase B/mammalian target of rapamycin (AKT/mTOR) pathway to induce autophagy, which plays a crucial role in regulating cellular energy balance. The gene discussed is PKN2; the disease is type 2 diabetes mellitus.