TRERF1 and Hyperglycemia: Confocal microscopy showed that, similar to hyperglycemia, hyperglycemia combined with shNetrin-1 significantly increased the proportion of RFP+GFP+ cells (P < 0.001), while the proportion of RFP+GFP- cells showing red fluorescence increased after the addition of autophagy activator Rapa on the basis of hyperglycemia alone.