Finally, we investigated the effects of SCF on the implantation potential of porcine blastocysts, especially focused research on the establishment efficiency of cloned ESCs, since this model has great application value in the study of human reproductive disease via generating patient-derived blastoid or gastruloids (8, 9), but its derivation efficiency is still low, unlike fertilized ESCs (39, 40). This evidence concerns the gene KITLG and reproductive system disorder.