Among these genes, nine were associated with gut microbiota that promotes ICC, including Veillonellaceae (TECPR2), Alistipes (TOP1MT and CAPZB), Enterobacteriales (KCNQ1), and Firmicutes (AMBP, NID2, CAB39, SPEF2, and FRMD4A). The gene discussed is FRMD4A; the disease is intrahepatic cholangiocarcinoma.