However, as a tumor-related huge protein family that has only been highlighted in recent years, in-depth molecular mechanistic studies on this family, especially regarding the regulation of EMT and downstream signaling pathways such as P53, MAPK/AKT, TGF-β by the AHNAK family are lacking and need to be further explored in the future with a view to providing new targets for tumor diagnosis and treatment. This evidence concerns the gene TGFB1 and neoplasm.