Suppressed HUVEC proliferation; considerably reduced endothelial cell migration and invasion, and capillary structure formation of HUVEC, downregulated VEGF-A; 50 mL Punarnavine significantly inhibited MMP-2 & MMP-9 expression in vitro; 15 mg/kg bw/d Punarnavine treatment decreased ascitic fluid (60.94%) & tumor (86.40%) volume in Ehrlich ascites model; reduced peritoneal angiogenesis exhibited anti-angiogenic property of Punarnavine. Here, MMP9 is linked to neoplasm.