To begin to identify the cell-autonomous factors driving insulin resistance, in previous studies, we have developed a unique model using induced pluripotent stem cell–derived (iPSC-derived) myoblasts (iMyos) taken from either type 2 diabetic patients and controls (15) or insulin-sensitive (I-Sen) and insulin-resistant (I-Res) nondiabetic individuals (16) and investigated basal and insulin-stimulated protein phosphorylation using quantitative global phosphoproteomics. This evidence concerns the gene INS and type 2 diabetes mellitus.