The glycoprotein EFEMP1 was one of the enriched proteins that was found in the 143B cell SCR, and in the plasma of mice with primary OS, lung metastasis or treated with the 143B cell-derived SCR in a tumour-free setting, suggesting a driving oncogenic role for lung metastasis in a premetastatic stage. This evidence concerns the gene EFEMP1 and neoplasm.