Certain gene clusters were associated with Amsel-BV diagnoses, especially gene cluster 10 (Fig. 7c), which is primarily comprised of genes from Gardnerella sp 11 and sp 13 (Fig. 7b) and encodes a vaginolysin (V1099403), a hemolysin (V1099398), the muralytic enzyme precursor Rpf2 (V1385511), a vancomycin resistance protein (V1099665), and glycogen debranching enzymes pullulanase (V1313000) and oligo-16-glucosidase (V1195401). This evidence concerns the gene RPF2 and bacterial vaginosis.