As illustrated in Fig. 1A, significant up-regulation of SIX1 was observed in 28 tumors, including breast cancer (Tumor: 2.62 ± 2.27, Normal:1.05 ± 1.77, P = 2.8e−29), while down-regulation was noted in 2 tumors. This evidence concerns the gene SIX1 and breast carcinoma.