We observed varying degrees of decrease in the fluorescence intensity of Oct4, Sox2, Aldh1a1, Epcam, and Itgb1 in tumor tissues formed by 66cl4-shSix1 KD1-luc and 66cl4-shSix1 KD2-luc compared to those formed by 66cl4-SCR-luc. This evidence concerns the gene POU5F1 and neoplasm.