Subsequently, PPI and GSEA assays indicated that genetic and cell biological aspects including CCNB1, MAD2L1, H2AFZ, CXCL2 genes and cell cycle, as well as several signal pathways including DNA sensing, NOD-like receptors, NOTCH and TGF-β signal pathways may be involved in CXCL3-mediated tumor biology in COAD. The gene discussed is CXCL3; the disease is colon adenocarcinoma.