In this study, the results from bioinformatics and immunohistochemistry assays exhibited that CXCL3 mRNA and protein are surprisingly upregulated in COAD tissues, and its high-level expression is closely correlated with several clinicopathological parameters, including patient’s clinical stage, race, gender, age, histological subtype, nodal mestastasis and TP53 mutation status. This evidence concerns the gene CXCL3 and colon adenocarcinoma.