ESR2 and breast cancer: While it may be theoretically possible that the NR5A motif, which is an extension of a half-ERE site, can be bound by ERβ, this is not supported since the NR5A motif was not found to be enriched in ERβ ChIP-seq of other cells that do not express LRH-1 (exogenous ERβ expressed in colon or breast cancer cell lines [34]).