While we have previously studied exogenously expressed human ERβ with the same antibody and revealed its chromatin binding pattern in non-reproductive cell types, including breast and colon cancer cell lines [34, 43], and others have reported endogenous ERβ cistromes but not using the validated antibody (rat male germ cells [36] and mouse endometrium overexpressing ERβ [37]), this is to our knowledge the first time the endogenous ERβ cistrome is described in detail using validated tools. Here, ESR2 is linked to colonic neoplasm.