To our knowledge, this observation has been consistent across every grade 4 astrocytoma cell line reported to date (both IDH-wild type and mutant), including LN229102, A172102, IDH1-R132H mutated U87MG103, and 9 molecularly diverse human lines spanning P53 and IDH-WT primary and recurrent lines from male and female patients104. This evidence concerns the gene IDH1 and astrocytoma (excluding glioblastoma).